Barbara Testoni

Barbara Testoni was born in Modena, Italy, where she graduated in Biotechnology in 2002. In 2005 she obtained her PhD in Genetics and Molecular Biology at the University of Milan, working on eukaryotic transcriptional regulation in the setting of skin development. Then, she moved to the University of Rome “La Sapienza”, where she began to work on liver disease and type-I interferon signaling. She particularly focused on the epigenetics mechanisms at the basis of Hepatitis C virus-driven Interferon stimulated genes (ISGs) deregulation and set-up a micro-chromatin immunoprecipitation (ChIP) technique to look for histone post-translational modifications in human liver biopsies. At present, she works as senior scientist at the Cancer Research Center of Lyon (CRCL), in the “Viral Hepatitis” team. Her research interests mainly include the investigation of the epigenetic mechanisms at the basis of host and viral gene regulation during HCV and HBV infections, with particular focus on the transcriptional regulation of the HBV minichromosome (cccDNA). She adapted the ChIP technique to specifically study HBV minichromosome epigenetics and she associated cccDNA-specific ChIP to Mass spectrometry analysis to un cover the proteome associated to HBV cccDNA in infected primary human hepatocytes. She is also involved in translational studies, to the aim of understanding the relationships between HBV persistence and intrahepatic innate immunity perturbations and to characterizing new serum biomarkers for intrahepatic cccDNA activity.

Presentations

How to impact cccDNA kinetics by new treatments ? Do we need cccDNA quantification or epigenetic studies? 10 April 2019 10:00 - 10:15